XB-IMG-172237
Xenbase Image ID: 172237
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Figure 1. An XlHbox I-Ab-Injected Tadpole with an Extended Hindbrain
Line diagrams at the top of the figure indicate a control uninjected tadpole (I) and a long XlHbox lAb-injected tadpole with an extended hindbrain
(II). The defect in the dorsal fin, by which this tadpole was originally identified, is shown in drawing II. A thick bar above each drawing indicates
the anteroposterior band of expression of the long XlHbox 1 protein in Xenopus embryos. This region is also shaded in the CNS; note that in control
tadpoles (I) it occupies spinal cord, but in the antibody-injected tadpole (II) it lies in a hindbrain-like structure complete with expanded fourth ventricle.
(A-E) (control, tadpole I) and (A’-E’) (antibody-injected, tadpole II) show transverse sections taken at the levels indicated by the vertical arrows
in drawings I and II. All sections were immunostained with long XlHbox IAb. The dorsal fin defect indicated in drawing II is visible in (C’) and (LY);
compare with (C) and (D). Animals injected with the out-of-frame control antibody (see text and Table 1) are indistinguishable from the uninjected
tadpole shown here. The position of the pronephros (PN) is shown. In (C) and (CT, the region of expression of long XlHbox 1 protein, which appears
as dark nuclei, is indicated by an arrowhead. Note that posterior to the region of XlHbox 1 expression, the CNS adopts the morphology of normal
spinal cord. Abbreviations: BA, branchial arch; DF, dorsal fin; EC, ependymal canal; HB, hindbrain; IV, the hindbrain cavity, called the fourth ventricle;
WC, myotome; NO, notochord; SC, spinal cord. Bar = 50 pm. Image published in: Wright CV et al. (1989) Copyright © 1989. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |