XB-IMG-172239
Xenbase Image ID: 172239
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Figure 3. Injection of Long XlHbox l-Ab Produces Spinal Cord Defects
over the Region of Expression of Long XlHbox 1 Protein
Three 2.5-day-old tadpoles are presented in pairs of photographs (A,
8; C. D; E, F). (A) and (6) show a tadpole injected with an out-f-frame
control antibody. (C)-(F) show two long XlHbox 1-Ab-injected tadpoles
with phenotypic defects over the cervical spinal cord. The left-hand
panel of each pair shows the embryo immunostained in whole mount
with the spinal cord-specific XlHbox 6 antibody. XlHbox 6-positive
nuclei in the spinal cord can be seen as dark brown dots over a uniform lighter-brown background. Over the region of spinal cord alteration in
the antibody-injected tadpoles, the CNS resembles hindbrain, being
closed dorsally by a thin roof and having a large cavity continuous with
the fourth ventricle. Note that the position indicated by the arrow, which
is within the region of the CNS affected by injecting long XlHbox lAb,
expresses XlHbox 6 protein. Also note the position of the pronephros
(PN) in relation to the normal spinal cord or morphologically transformed
region of spinal cord, and the region of XIHbox 6 immunostaining.
The right-hand panel of each pair shows a transverse section of
the same embryo immunostained with long XlHbox 1Ab. The section
was taken at the level indicated by the arrow in (A), (C), or(E), as appropriate.
In the sections, the dark purple nuclear staining in the CNS
shows that long XlHtxrx 1 protein is expressed in the cervical spinal
cord of control tadpoles (B), but in the hindbrain-like regions of
antibody-injected animals (D and F). Abbreviations: EC, ependymal canal:
IV, hindbrain cavity called the fourth ventricle; SC, spinal cord. Image published in: Wright CV et al. (1989) Copyright © 1989. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |