XB-IMG-172364
Xenbase Image ID: 172364
|
Fig 7. Wnt activity during retinogenesis.
(A) Whole-mount gfp in situ hybridization on Tg(pbin7Lef-dGFP) embryos from stage 14 to stage 20. Anterior views with the eye fields (dotted lines) are shown. (B) In situ hybridization against gfp on stage 21 to stage 40 Tg(pbin7Lef-dGFP) embryos. Whole-mount (lateral views of the head, anterior to the left) and transverse retinal sections are shown. Arrowhead points to surface ectoderm, black arrows to the Retinal Pigmented Epithelium (RPE) and presumptive RPE (pRPE), white arrows to the Ciliary Marginal Zone (CMZ). Black dotted lines delineate the optic vesicle. White dotted lines delineate the boundary between neural versus non-neural territories in the retina. (C) Schematic of a CMZ showing its spatial organisation with stem cells closest to the periphery (region 1), proliferative retinoblasts in the middle (region 2) and postmitotic cells at the central edge (region 3). On the bottom, an enlargement of the region delineated with black dotted lines in the stage 40 retinal section image shows the gfp signal in the peripheral half of the CMZ. White dotted lines delineate the 3 zones depicted in the schema. A strong staining is observed in zone 1, a fainter staining is detected in zone 2 and barely no staining is observed in zone 3. CMZ: Ciliary Marginal Zone; L: lens; NC: migrating neural crest cells; ov: optic vesicle; pNR: presumptive Neural Retina, pRPE: presumptive Retinal Pigmented Epithelium. Image published in: Borday C et al. (2018) © 2018 Borday et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |