Xenbase Image ID: 172385

Figure 3. ERK3 is required for pronephros and epidermal development in Xenopus laevis embryos. A-G, Phenotypes of ERK3 knockdown embryos. Lateral views with anterior to the left and dorsal up. A-E, Control MO (60 ng in (A), 40 or 80 ng in (B), 15 ng in (C) and 40 ng in (E)), ERK3 MO1/2 (30 (A) or 7.5 (C) ng each of ERK3 MO1 and ERK3 MO2) or ERK3 MO3 (40 or 80 ng in (B) and 40 ng in (E)) was co-injected with the lineage tracer dextran-fluorescein into both (A, B) or the left (C, E) V2 blastomere(s) at the 8-cell stage. Embryos with pronephric fluorescence were further analyzed. A, B, Phenotypes at stage 42. The black arrowhead indicates edema. The graph indicates the percent of embryos with edema from four (A) or two (B) independent experiments. **P<0.01 by z-test. Scale bars, 1 mm. C-E, Expression of the pronephric marker gene atp1b1 at stage 33/34. pn, pronephros. Scale bars, 200 m. C, D, In the rescue experiment, Myc-ERK3A and Myc-ERK3B mRNAs (1 ng each) were co- injected with ERK3 MO1/2. D, Percent of embryos with normal or aberrant atp1b1 expression in pronephros in four independent experiments. Severe, no expression; Mild, weak expression. **P<0.01 by Mann-Whitney U test. E, Expression of atp1b1 was inhibited by ERK3 MO3 (N=23/23) but not by control MO (N=1/28) in three independent experiments. F, G, Control MO (60 ng), ERK3 MO1/2 (20 ng each of MO1 and MO2) or ERK3 MO3 (60 ng) was injected into the animal regions of ventral blastomeres at the 4-cell stage. In three independent experiments, the epidermal architecture was disrupted by ERK3 MO1/2 (N=14/38 at stage 23 and N=37/38 at stage 39) and ERK3 MO3 (N=36/40 at stage 39) but not by control MO (N=0/39 at stage 23 and 39). Black arrowheads indicate initially observed, local disintegration of the epidermis. Scale bars, 500 m. Image published in: Takahashi C et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue atp1b1.L atp1b1a, Beta1-Na/K-ATPase, Na+-K+-ATPase, β1-NaK-ATPase X. laevis Throughout NF stage 33 and 34 pronephric kidney somite Image source: Published Larger Image Printer Friendly View

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