XB-IMG-172395
Xenbase Image ID: 172395
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Figure 13. Phenotypes induced by ERK3 knockdown are partially rescued by tfap2a overexpression in Xenopus laevis. A, B, Control MO (20 ng) or ERK3 MO1/2 (10 ng each of MO1 and MO2) was injected with or without tfap2a.L mRNA (400 pg) into the left V2 blastomere at the 8- cell stage. All injections were carried out using dextran-fluorescein as a lineage tracer. Embryos with pronephric fluorescence were fixed at stage 33/34 for whole mount in situ hybridization. A, Lateral views with anterior to the left and dorsal up. pn, pronephros. Scale bars, 200 m. B, The percent of embryos with normal or aberrant atp1b1 expression in pronephros was scored in five independent experiments. Severe, no expression; Moderate, weak and discontinuous expression; Mild, weak but continuous expression. *P<0.05 by Mann-Whitney U test. C, D, Control MO (40 ng) or ERK3 MO1/2 (20 ng each of MO1 and MO2) was injected with or without tfap2a.L mRNA (800 pg) into the animal regions of both ventral blastomeres at the 4-cell stage. CAAX-GFP mRNA (200 pg) was also co-injected in all conditions to visualize the plasma membrane. Injected embryos were fixed at stage 13 for immunofluorescence using anti-E-cadherin. Apical surfaces of the epidermal epithelia were observed by confocal microscopy. Scale bars, 10 m. D, Each point represents the average fluorescence intensity of junctional E-cadherin in one cell (control MO, 49 cells from 16 microscopic fields; ERK3 MO1/2, 51 cells from 20 microscopic fields; ERK3 MO1/2 plus tfap2a.L mRNA, 58 cells from 19 microscopic fields; from two independent experiments). The bars represent the average±S.D. *P<0.05 by Mann- Whitney U test. Image published in: Takahashi C et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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