Xenbase Image ID: 172471

Figure 2. Hyperaldosteronism mutations in Xenopus Na/K pumps. (A) TEVC recording at −50 mV from a Na+-loaded oocyte expressing wild type. Application of 10 mM K+ in NMG+o stimulated outward current. There is zero ouabain (ouab)–sensitive steady-state current in Na+o alone. (B) A similar TEVC recording from an oocyte expressing L104R, 3 d after cRNA injection. K+o-induced outward current was absent, and switching from NMG+o to Na+o induced an inward current that was partially inhibited by perfusion of 10 mM ouabain. Vertical deflections along the current trace represent 100-ms voltage pulses to obtain I-V curves. (C) Mean ouabain-sensitive I-V plots measured in NMG+o (filled symbols) and in Na+o (open symbols), 3–4 d after injection, from oocytes expressing L104R (down triangles), V332G (circles), delF100-L104 (diamonds), and EETA963S (up triangles). Number of experiments is indicated in parentheses. Error bars represent SEM. Image published in: Meyer DJ et al. (2017) © 2017 Meyer et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page