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Figure 7. Turnover rates of wild-type and mutant human Na/K pumps. (A) Representative recording from an oocyte expressing wild-type pumps, held at −50 mV, in which application of 3 mM K+o in NMG+o induced outward current. (B) Representative recording from an oocyte expressing L104R pumps in which replacing NMG+o with Na+o solution induced inward current. The quantity of [3H]ouabain bound to the oocytes in A and B is also indicated. (C) Bar graph showing mean turnover rates (moles of charge per second/moles of [3H]ouabain bound; i.e., s−1) measured in individual oocytes, for the outward current (bracketed as “OUT”) in oocytes expressing wild type (n = 17) and for the Na+o-induced inward current (bracketed as “IN”) in oocytes expressing L104R, V332G, delF100-L104, and EETA963S. Measurements were performed 4–5 d after injection, except for delF100-L104, which was performed 3 d after injection because of large currents. Note the break along the y axis. The number of experiments is indicated in parentheses. Error bars represent SEM. Image published in: Meyer DJ et al. (2017) © 2017 Meyer et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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