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Fig. 6. Expression analysis of uncharacterised genes identified by the means of nongenetic axis perturbation and comparative transcriptomics. RT-qPCR (A) and WMISH (B) of c8orf4, Xetrov72022004, K00726 and cpe. (A) Error bars, SEM of biological triplicates. Student's two-tailed t-test: *, p < 0.1; **, p < 0.05; and ***, p < 0.01. (B) Mid-gastrula stages, vegetal and lateral view except for cpe with vegetal view only; and mid-tailbud stage, lateral view. Image published in: Monteiro RS et al. (2018) © 2018 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tcim c8orf4, tc-1, tc1 X. tropicalis Throughout NF stage 11 ectoderm cpe carboxypeptidase E X. tropicalis Throughout NF stage 11 marginal zone dorsal marginal zone tcim c8orf4, tc-1, tc1 X. tropicalis Throughout NF stage 28 ectoderm head cpe carboxypeptidase E X. tropicalis Throughout NF stage 28 central nervous system spinal cord cranial nerve neural tube posterior neural tube XB22041735.L K00726 X. laevis Throughout NF stage 11 upper blastopore lip dorsal dorsal marginal zone XB22041731.L LOC100486385, Xetrov72022004 X. laevis Throughout NF stage 11   XB22041735.L K00726 X. laevis Throughout NF stage 26 central nervous system brain spinal cord XB22041731.L LOC100486385, Xetrov72022004 X. laevis Throughout NF stage 29 and 30 head Image source: Published Larger Image Printer Friendly View

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