XB-IMG-172570
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Fig. 7.
Ectopic right-side Pitx2c induces left liver lobe morphology. Synthetic mRNA encoding dexamethasone (Dex) inducible Pitx2c (Pitx2cGR) was targeted to the mesoderm surrounding the right liver diverticulum (see Fig. 8A-B). Liver morphology was then analyzed in un-induced controls (-Dex) and Dex-induced (+Dex) embryos at NF 46 using in situ hybridization with a probe for fibrinogen. In un-induced controls (A), the right (R, orange outline) lobe is larger and more elongate than the left lobe (L, yellow outline). However, in Dex-induced embryos (B-C), the right lobe of the liver is smaller, similar to control left lobe morphology (i.e., the liver exhibits left isomerism; L-Iso). Scale bars= 200 µm. D) Quantification of liver phenotypes (see cartoons) in un-induced (Pitx2cGR -Dex) and induced (Pitx2cGR +Dex) embryos. E) As expected in un-induced embryos (-Dex), the right lobe of the liver has a greater gross length to width ratio than the left lobe (NF 46). However, in embryos with ectopic right-sided Pitx2c activity (+Dex), the right lobe is smaller, with a decreased length to width ratio comparable to that of left lobes. Error bars = S.E. Asterisks indicate p < 0.0001 (**); NS, not significant. Image published in: Womble M et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
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