XB-IMG-172575
Xenbase Image ID: 172575
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FIGURE3 Phenotypes of XCXCLh- and XCXCLh- MO- injected embryos. (a) The specificity of the antisense morpholino oligonucleotides for XCXCLh. Western blot analysis of the injected embryos at stage 10. XCXCLh- MO inhibited the translation of FLAG- tagged mRNA containingthetargetedsite.ThetranslationoftheFLAG-taggedmRNAcontaining5-mismatchedsequencesatthetargetedsitewasnot inhibited by the XCXCLh- MO. β- globin is used as a loading control. (b) XCXCLhmRNA(500pg/blastomere)orXCXCLh- MO (10 ng/blastomere) was injected into the marginal region of two dorsal blastomeres of 4- cell embryos. Gastrula, stage 12 (vegetal view). Neurula, stage 16 (dorsal view). Tailbud, stage 30 (lateral view); left, weak phenotypes; right, severe phenotypes). WISH of Chordin, whole mount in situ hybridization of bisected embryos (stage 11) with using Chordin probes. Arrowheads indicate expression of Chordin. (c, d) Effects on phenotype by increasing doses of XCXCLh (c) and XCXCLh-MO(d).Theratioofeachphenotypeintheinjectedembryos(45embryos/bar)was indicated in the graph. Black bars represent ratio of severe phenotypes as shown in B, right panels. Gray bars represent ratio of weak phenotypes as shown in B, left panels. White bars represent normal embryos Image published in: Goto T et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |