XB-IMG-172577
Xenbase Image ID: 172577
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FIGURE5 The role of XCXCLh in differentiation. (A and B) Phenotypes of XCXCLh- injected embryos at stage 23. (a) Control. (b) XCXCLhmRNA(500pg/blastomere) was injected into the dorsal animal blastomeres of 8- cell embryos. (c and d) Animal caps of the injected embryos. (c) Control. (d) XCXCLh mRNA (500pg/blastomere)wasinjectedintoall animal blastomeres of 8- cell embryos, and animal caps were dissected at stage 8andcultureduntilstage25.(e)RT-PCR analysis of the animal caps of the XCXCLh- injected embryos. Total RNA was extracted from the animal caps of stage 10(leftpanels)andstage25(rightpanels)embryos. (f) RT- PCR analysis of the XCXCLh- MO- injected embryos. XCXCLh- MO (10 ng/blastomere) was injected into the vegetal region of all blastomeres of 4- cell embryos. Total RNA was extracted from the embryos (stage 16) Image published in: Goto T et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |