Xenbase Image ID: 172578

FIGURE6 Effects of XCXCLh on the anterior- posterior axis formation. (a–c) Phenotypes of tBR and XCXCLh co- injected embryos. (A) Control, stage 40 embryo. (b) The tBRmRNA(500pg/blastomere) was injected into the ventral blastomeres of 4- cell embryos. (c) The tBR (500pg/blastomere)andXCXCLh(500pg/blastomere) mRNAs were co- injected. (d) RT- PCR analysis of animal caps at the neurula stage (stage 16). The tBR(500pg/blastomere) and different dose of XCXCLh (+,125pg;++,250pg;+++,500pg/blastomere) mRNAs were co- injected into the animal blastomeres of 8- cell embryos. Total RNA was extracted from the animal caps (stage 16). (e) RT- PCR analysis of animal caps at the gastrula stage (stage 10). The indicated Xenopus CXC- type receptormRNAs(500pg/blastomere)were injected into the animal blastomeres of 8- cell embryos. Total RNA was extracted at stage 10. (f) The different dose of XCXCLh(+,125pg;++,250pg;pg/blastomere) mRNAs were co- injected with indicated Xenopus CXC- type receptor (+,125pg;++,250pg;pg/blastomere)mRNAs into the animal blastomeres of 8- cell embryos. Total RNA was extracted at stage 10 Image published in: Goto T et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page