Xenbase Image ID: 172615

Fig. 2. anos1 is a target of Pax3 and Zic1. (A) Injection of pax3MO (40 ng) or zic1MO (40 ng) reduces anos1 expression at the neurula stage. Dorsal views, anterior to top. The injected side is to the right as indicated by the presence of the lineage tracer (Red-Gal). The graph indicates the percentage of embryos with normal (white) or reduced/lost (red) anos1 expression. The number of embryos analyzed is indicated on top of each bar. (B) mRNA encoding pax3GR and zic1GR (100 pg each), alone or in combination were injected into both blastomeres in the animal pole at the 2-cell stage. At the blastula stage (stage 9), animal cap (AC) explants were dissected and cultured for 8 h in the presence of dexamethasone (+dex). (C) anos1 and snai2 expression in pax3GR and zic1GR injected AC explants analyzed by qRT-PCR. Image published in: Bae CJ et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue anos1.L kal1 X. laevis Sometime during NF stage 14 to NF stage 17 neuroectoderm neural plate neural crest neural plate border Image source: Published Larger Image Printer Friendly View

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