XB-IMG-172616
Xenbase Image ID: 172616
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Fig. 3. Anos1 is required for neural crest formation. (A) Western blot of embryos injected with mRNA encoding a myc-tagged version of Xenopus anos1, alone or in combination with increasing doses of anos1MO, 10 ng (+), 20 ng (++), and 40 ng (+++), showed that anos1MO blocks Anos1 protein accumulation. (B) Unilateral injection of anos1MO (40 ng) at the 2-cell stage causes a reduction/loss of expression of snail2 and sox10, and a lateral expansion of sox2 expression domain. Injection of Xenopus anos1 mRNAs (3 ng) did not significantly affect snail2 and sox10 expression levels, although their expression domain was shifted laterally. The expression of sox2 was only marginally expanded in these embryos. Dorsal views, anterior to top. The injected side is to the right (Red-Gal). (C) The graphs indicate the percentage of embryos with normal (white), reduced/lost (red) expression. The number of embryos analyzed is indicated on top of each bar. (D) In explants, the induction of snail2 and sox8 by co-injection of noggin (200 pg) and wnt8a (10 pg) mRNA is dramatically reduced in the context of embryos injected with anos1MO (40 ng). This reduction in neural crest genes expression is associated by an increase in sox2 expression. The values were normalized to ef1α. A representative experiments is shown from three independent experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) Image published in: Bae CJ et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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