Xenbase Image ID: 172618

Figure 2. Four candidates interact with HNF1bbb in GST pull-down assays. Full-length interaction partners with an N-terminal myc-tag were produced in a rabbit reticulocyte system (TNT, Promega) and incubated with the recombinant GST–HNF1bbb fusion protein bound to glutathione sepharose beads. After washing, proteins were recovered and detected by 9E10 mouse anti-myc antibody in Western blots. As controls, GST bound to sepharose or sepharose alone were used. Image published in: Dudziak K et al. (2008) Copyright © 2008. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page