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Figure 6. The effect of E4F1 and ZFP36L1 on the transactivation potential of HNF1β. HEK293 cells were transfected with the luciferase reporter construct driven by the osteopontin (OPN), Ksp-cadherin or the artificial promoter containing four HNF1-binding site linked to the tk promoter (HNF14-tk). The expression vector Rc/CMV-HNF1β and either (a) pCS2+mt-E4F1 or (b) pCS2+mt-ZFP36L1 were included in the transfection as indicated. The luciferase activity without expression vector was used as standard. The standard deviation from a least six measurements are given and significant activation (P-value <0.05) by the transcription factors alone are indicated by the corresponding P-value. The bracket in B indicates the significant downregulation by ZFP36L1 (P-value 1.9 × 10−5). The ACE231 and pkhd113 promoter were also affected by E4F1 and ZFP36L1 in the absence of HNF1β (data not shown Image published in: Dudziak K et al. (2008) Copyright © 2008. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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