Xenbase Image ID: 172646

Fig. S4. Significant correspondence between RSF1 and RNF2/Ring1B regulated genes. (A) Immunoblot analyses of HeLa cells with KD of RSF1 or RNF2 confirmed the reduction of corresponding target proteins. Antibodies used are indicated on the right of the panels. (B) Waterfall plot showing gene expression changes in RNF2 KD and RSF1 KD HeLa cells. The majority of these genes show changes in the same direction. (C) Immunoblot analyses of control, RSF1, RNF2, and SNF2H KD HeLa cells confirmed the reduction of corresponding target proteins. Antibodies used are indicated on the right of the panels. (D) RT-qPCR analysis of the expression of representative genes in control HeLa cells and in HeLa cells with KD of RSF1, RNF2, and SNF2H. (E) Immunoblots of wild-type, RSF1 KO, or Ring1B KO mouse ESCs confirmed the reduction of corresponding target proteins. Antibodies used are indicated on the right of the panels. (F) Waterfall plot showing gene expression changes in Ring1B KO and in RSF1 KO mouse ESCs. The majority of these genes show changes in the same direction. (G) Venn diagram shows the overlap of H2Aub bound genes with significant changes in expression in Ring1B or RSF1 KO mouse ESCs. Image published in: Jones AE et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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