Xenbase Image ID: 172677

Fig. 2. AChE is required for endoderm cell rearrangement and polarization. In NF 46 intestine sections of embryos injected with control MO (A), AChE MO (B), AChE MO plus wt AChE mRNA (C), or AChE MO plus mutAChE mRNA (D), β-catenin (red) outlines membranes of injected (GFP labeled, green) and uninjected cells. Serial sections from embryos in A-D were immunostained for integrin (green) and aPKC (red) (E-H); the boxed regions are shown at higher magnification in I-L. A single-layer columnar epithelium of polarized cells forms in control MO-injected intestines (I). AChE MO-injected cells (J) are rounder (asterisks), unpolarized [absence of aPKC (red), arrowhead] and fail to form a single layer. Defects are rescued by co-injection with wt AChE mRNA (K) or mutAChE mRNA (L). Scale bars: 100 μm in A-H; 25 μm in I-L. Image published in: Pickett MA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Throughout NF stage 46 endoderm intestine plasma membrane prkcz.L PKC zeta, pkc-zeta, PKCzeta, PKMz, TPKC X. laevis Throughout NF stage 46 intestine plasma membrane itgb1.L Beta 1 integrin, cd29, fnrb, gpiia, itgb1-a, itgb1-b, mdf2, msk12, vla-beta, vlab X. laevis Throughout NF stage 46 intestine microtubule Image source: Published Larger Image Printer Friendly View

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