XB-IMG-172678
Xenbase Image ID: 172678
|
Fig. 3.
AChE is required for microtubule organization and endoderm differentiation. In NF 46 intestine sections of embryos injected with control MO (A), AChE MO (B), AChE MO plus wt AChE mRNA (C), or AChE MO plus mutAChE mRNA (D), β-catenin (red) outlines cell membranes of injected (GFP labeled, green) and uninjected cells. Serial sections from embryos in A-D were immunostained to visualize microtubules (α-tubulin, green) (E-H); boxed regions are shown at higher magnification in I-L. Microtubules are apically enriched and oriented along the apical-basal axis of columnar epithelial cells (I). Microtubule organization is disrupted when AChE is knocked down (J), but is rescued by co-injection of the AChE MO with wt AChE mRNA (K) or mutAChE mRNA (L). Serial sections from embryos in A-H were immunostained for E-cadherin (green) and IFABP (red) (M-P); boxed regions are shown at higher magnification in Q-T. IFABP is expressed in control MO-injected intestine (Q). AChE knockdown (R) prevents differentiation of the endoderm, as indicated by the absence of IFABP in AChE MO-injected cells. Differentiation is restored by co-injection of the AChE MO with wt AChE mRNA (S) or mutAChE mRNA (T). Nuclei, blue (TO-PRO-3). Scale bars: 100 μm in A-H,M-P; 25 μm in I-L,Q-T. Image published in: Pickett MA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Image source: Published Larger Image Printer Friendly View |