Xenbase Image ID: 172680

Figure S1) Morpholino-mediated knockdown of AChE protein expression. In sections through the intestine of NF 46 embryos injected with control MO (A), AChE MO (B), AChE MO + wt AChE mRNA (C), or AChE MO + mutAChE mRNA (D), β-catenin (red) outlines membranes of injected (i.e., GFP labeled; green) and uninjected cells. Serial sections from embryos in A-D were immunostained for AChE (red) and images of the boxed regions are shown at higher magnification (E-H). Apical localization of AChE protein (indicated by arrowheads in E-H) is observed in control MO injected cells (E), but is missing in AChE MO injected epithelium (F). AChE staining is rescued by co-injection with either wt AChE mRNA (G) or mutAChE mRNA (H). Scale bars = 100 μm (A-D); 25 μm (E-H). Image published in: Pickett MA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Throughout NF stage 46 intestine plasma membrane ache.L acetylcholinesterase, arache, n-ache X. laevis Throughout NF stage 46 intestine plasma membrane gut epithelium Image source: Published Larger Image Printer Friendly View

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