Xenbase Image ID: 172685

Figure S6) AChE activity is not required for endoderm cell-cell adhesion. A-E’) Endoderm cells were dissociated from four NF 41 tadpoles (A-E) previously exposed in vivo from NF 33-41 to DMSO (A,A’), Malathion (B,B’), Chlorpyrifos-methyl (C,C’), Carbachol (D,D’) or Atropine (E,E’). Dissociated cells were allowed to re-aggregate for 60 minutes (60’) after reintroduction of Ca2+ ions into the media (0’). Organophosphate inhibition of AChE (B-C’), chemical stimulation of cholinergic receptors (D,D’), or antagonism of cholinergic receptors (E,E’) had no effect on re-aggregation (A’-E’), indicating cell-cell adhesion is independent of AChE or cholinergic signaling. At least three separate assays were performed per chemical. F-J’) Endoderm cells were dissociated (F-J) from four NF 41 tadpoles and subsequently exposed ex vivo to DMSO (F,F’), Malathion (G,G’), Chlorpyrifos-methyl (H,H’), Carbachol (I,I’), or Atropine (J,J’) during a 60 minute re-aggregation assay. Organophosphate inhibition of AChE (G-H’), chemical stimulation of cholinergic receptors (I, I’), or antagonism of cholinergic receptors (J, J’) had no effect on re-aggregation (F’-J’), indicating cell-cell adhesion is not mediated by AChE or cholinergic signaling. Image published in: Pickett MA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page