Xenbase Image ID: 172696

Fig. S2. Pfkfb4 is specifically upregulated in the neural border and neural crest during neurulation. (A-J). Transverse sections through the anterior neural plate at mid and late neurula stages (stage 14 and 18, respectively) allow comparing Snail2 and Pfkfb4 expression in the neural crest (Snail2 and Pfkfb4), neural plate (Pfkfb3) and somites (Pfkfb1). (A,F): The notochord, neural plate and paraxial mesoderm are outlined on hematoxylin-eosin stained paraffin sections. (B-E,G-J) Vibratome sections of WISH-stained embryos. The arrow indicates the midline and the notochord. The blue arrowheads indicate in situ hybridization staining position. Red bar positions the neural border at stage 14. Scale bar = 200μm. (K) A quantitative analysis of Xenopus laevis Pfkfb1-4 expression levels shows that Pfkfb4 is specifically enriched at the neural border of frog neurulas (red), compared to the anterior neural fold (yellow). All values are normalized to Odc expression and to the average expression in a sibling whole embryo lysed at late neurula stage 18 (blue, value = 1). Snail2 expression is used to monitor the quality of dissected tissues and shows high and specific expression at the neural border. Image published in: Figueiredo AL et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 14 neural plate border neural crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 18 neural crest neural plate border pfkfb4.L X. laevis Throughout NF stage 14 neural crest neural plate border pfkfb4.L X. laevis Throughout NF stage 18 neural plate border neural crest pfkfb3.L X. laevis Throughout NF stage 14 neural plate pfkfb3.L X. laevis Throughout NF stage 18 neural plate pfkfb1.L LOC108704801 X. laevis Throughout NF stage 14 presomitic mesoderm pfkfb1.L LOC108704801 X. laevis Throughout NF stage 18 somite Image source: Published Larger Image Printer Friendly View

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