XB-IMG-172706
Xenbase Image ID: 172706
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Fig. 2. KDM3A is required for the primary
neurogenesis in Xenopus. (A) A schematic
showing the experimental design for B and C.
(B,B′) Embryos at stage 24/25 stained with red-Gal
and in situ hybridized with tubb2b. cMO or 3A MO
(40 ng) was injected into one cell at the two-cell
stage. The 3A MO-resistant kdm3a mRNA (300 pg)
together with 200 pg β-gal mRNAwas subsequently
injected into the 3A MO-injected cell at the two-cell
stage. The expression levels of tubb2b were
classified into three categories: ‘normal’, as seen in
cMO-injected embryos; ‘partial’, as seen in some of
KDM3A depleted embryos; and ‘complete’ loss, as
seen in the remainder of the KDM3A-depleted
embryos. Injection of the MO-resistant kdm3a
mRNA partially rescues the expression of tubb2b in
KDM3A morphants. The numbers on the top of
histograms in B′ are sums from two independent
experiments. (C,C′) Embryos at stage 16/17 stained
with red-Gal and in situ hybridized with tubb2b. cMO
and 3A MO (40 ng) were injected into one cell at the
two-cell stage. mRNA (100 pg) encoding neurog2
or ascl1 together with 200 pg β-gal mRNA was
subsequently injected into the MO-receiving cell at
the two-cell stage. Phenotypes were classified into
four categories based on whether ectopic neurons
were induced and/or whether tubb2b was ‘partially’
or ‘completely’ lost in the MO-injected side. The
numbers on the top of histograms in C′ are the total
number of samples from two independent
experiments. Image published in: Lin H et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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