XB-IMG-172707
Xenbase Image ID: 172707
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Fig. 3. Neurog2 fails to transactivate its neuronal
targets in KDM3A morphant embryos. (A) Embryos at
stage 17/18 in situ hybridized with Xenopus neurog2.
cMO or 3A MO (80 ng) was injected into both cells at the
two-cell stage. (B) qPCR results showing that KDM3A
depletion differentially affects the expression of neural
progenitor genes (sox2 and neurog2) and neuronal
genes (neurod1 and tubb2b). ns, no significance.
***P<0.001. cMO or 3A MO (80 ng) was injected into
both cells at the two-cell stage. Embryos were processed
for RT-qPCR at stage 16. (C,C′) Embryos at stage 18
stained with red-Gal and in situ hybridized with neurod1.
cMO or 3A MO (40 ng) was injected into one cell at the
two-cell stage. mRNA encoding Neurog2 (100 pg)
together with 200 pg β-gal mRNA was subsequently
injected into the MO-receiving cell at the two-cell stage.
(D,E) ChIP-qPCR detection of H3K27ac (D) and
H3K4me3 (E) at the neurod1 and tubb2b promoters.
cMO or 3A MO (80 ng) was injected into one-cell stage
embryos. mRNA encoding Neurog2 (100 pg) was
injected into both cells of half of the MO-injected
embryos at the two-cell stage. ef1α expression was
examined as a negative control. Locations of the qPCR
primers are schematically shown in the x axes. The
ChIP-qPCR experiments shown in D,E were combined
from two independent repeats. Three technical
replicates were made in each independent repeat.
**P<0.01; ***P<0.005; ns, not significant (according to a
two-tailed Student’s t-test). Image published in: Lin H et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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