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Fig. S2 (related to Fig. 2). Assessment of the effects of KDM3A depletion on
neural development.
(A, B, C) Control (cMO) and KDM3A morphant (3A MO) embryos at stage 18in situ
hybridized with indicated marker genes. (A) Neuronal genes. (B) Markers for neural progenitors (sox3, pax6) and the anteroposterior pattern (rx, krox20, en2, and hoxb9).
(C) Markers for neural plate border, neural crest specification and epidermis. (D)
Dorsal view of embryos at stage 18 showing that KDM3A depletion by injecting 60
ng 3A MO delays neural tube closure, which was partially rescued by injecting back
of 500 pg the MO-resistant kdm3a mRNA.