Xenbase Image ID: 172717

Fig. S4 (related to Figs. 3 and 4). Assessment of functional and physical interactions between KDM3A and Neurog2. (A) WISH detection of myt1 in embryos at stage 18 (dorsal view). cMO and 3A MO: 80 ng. neurog2 mRNA:100 pg. (B) Embryos at stage 15/16 in situ hybridized with tubb2b (dorsal view). 3A MO and cMO: 80 ng. RA (1.0 M) was added to culture medium from stage 12 to stage 15/16. (C) Two different types of cells (RA-induced NE-4C cells and human induced-Neu neural stem cells) were lysed and subjected to CoIP for detecting interaction between the endogenous KDM3A and Neurog2. A KDM3A antibody was used to pull down endogenous KDM3A protein, and a Neurog2 antibody was used to detect the signal of endogenous Neurog2 protein. Image published in: Lin H et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue myt1.L X-MyT1, xmyt1, XMyTI X. laevis Throughout NF stage 18 neural plate neuron Image source: Published Larger Image Printer Friendly View

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