XB-IMG-172720
Xenbase Image ID: 172720
|
Fig.S7 (related to Figs. 5 and 6). KDM3A facilitates the chromatin binding of
Neurog2.
(A, B) Anti-H3K9me2 ChIP-qPCR analyses indicating that overexpression of wild
type 6MT-KDM3A (1 ng) but not a catalytic mutant 6MT-KDM3A (H1130Y, 1 ng)
reduced the expression levels of H3K9me2 on the promoter regions of neurod1 (A)
and tubb2b (B). (C, D) Anti-H3K27ac ChIP-qPCR analyses indicating that ectopic
6MT-KDM3A or a catalytic mutant 6MT-KDM3A (H1130Y) did not alter the
H3K27ac marks on the promoter regions of neurod1 (C) or tubb2b (D). (E)
Anti-H3K9me2ChIP-qPCR analyses showing 3A MO (80 ng) increased the
H3K9me2 marks on the promoter region of tubb2b. (F) Anti-Myc ChIP-qPCR
analyses indicating that 80 ng 3A MO but not cMO blocked the overexpressed
6MT-Neurog2 from accessing the promoter of tubb2b. Image published in: Lin H et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |