Xenbase Image ID: 172734

Figure 2. Strap‐deficient EBs are unable to differentiate into primary germ layers. A. Phase‐contrast images of EB morphology during EB formation from Strap WT and KO ESCs at the indicated time points. Scale bar = 600 um. B. The cell cycle distribution of D10 EBs from WT and KO ESC lines was analyzed by FACS. The percentages of cell populations at each cell cycle phase are labeled. C‐E. qPCR analyses of lineage‐specific gene’ expression at Day 0, Day 4, Day 8 and Day 12 during EB formation of two independent WT and KO ESC lines. Error bars represent mean ±s.d. Each experi‐ ment was replicated at least three times. Image published in: Jin L et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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