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Figure 4. Loss of STRAP results in increased CYP26A1 mRNA and protein levels during EB differentiation. A. RT‐qPCR analyses of RA metabolic gene expression at Day 10 during EB formation.  Bars represent mean ±s.d., n=3, **P<0.01, when compared with the control.  B. RT‐qPCR analyses of Cyp26A1 expression at Day 4, Day 6, Day 8 and Day 10 dur‐ ing EB formation. Each point represents mean ±s.d., n=3.  C. Western blot analyses of the level of CYP26A1 in the in‐ dicated EBs at Day 10 and Day 12. β‐Actin was used as a loading control. D. Western blotting was used to determine the expression of STRAP protein in the control and STRAP knockdown E14 polyclonal cells. E. Levels of CYP26A1 and STRAP in EBs derived from shSTRAP and shCtrl clones (E14) were determined by western blotting using β‐Actin as a loading control.  F. 8‐day‐old EBs were treated with CYP26A1 inhibitor, liarozole (50 uM), for 48 hours and total RNA from control and knockdown EBs was subjected to RT‐qPCR analyses for detecting RA‐targeted genes. No significance between the two groups was observed. Bars represent mean ±s.d., n=3. Image published in: Jin L et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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