XB-IMG-172737
Xenbase Image ID: 172737
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Figure 5. Krüppel‐like factor 9 (KLF9) and Sp1 coordinately regulate Cyp26A1 transcription in STRAP‐depleted EBs.
A. Either E14 control or shSTRAP cells were transfected with the indicated Cyp26A1 luciferase reporters. Luciferase
activity was normalized to ‐Gal activity and presented as mean ±s.d., n=3, *P<0.05, when compared with the corre‐
sponding control. B. Western blots of total cell lysates from indicated EBs with STRAP knockdown were analyzed for
KLF9 and Sp1 expression. C. E14 WT cells were co‐transfected with the indicated Cyp26A1 promoter reporter as well
as Sp1 or KLF9 expression plasmids. Luciferase activity was normalized to ‐Gal activity and presented as mean ±s.d.,
n=3, *P<0.05, **P<0.01. D. The murine Cyp26A1 promoter showing the positions of potential KLF9 binding sites
(black boxes) and the transcription start site (TSS). Black lines indicate the amplified regions for ChIP primers. E. ChIP
assays were performed from cells mentioned above using anti‐KLF9 or anti‐Sp1 antibody. PCR amplification was per‐
formed within the target region of the Cyp26A1 promoter. Results are expressed as percentages of immunoprecipi‐
tated DNA compared to total input DNA. *P<0.05, ** P<0.01, when compared with the corresponding control. Image published in: Jin L et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |