XB-IMG-172738
Xenbase Image ID: 172738
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Figure 6. STRAP knockdown influences anterior‐posterior neural patterning and impairs the body axis and eye de‐
velopment in early Xenopus embryogenesis. A. Strap transcript was detected in the entire blastopore (a), early neu‐
rulation (b), late neurula stage (c), and the tailbud and the tadpole stages (d and e, respectively). B. 50 ng Strap‐MO
was injected into the animal regions of two‐cell‐stage embryos and resulted in defects in the tadpole stage. C. Strap
RNA (0.20 ng) was injected alone or with 25 ng Strap‐MO unilaterally into two‐cell‐ stage embryos. The embryos were
stained with the β‐Gal substrate Red‐Gal. D. 50 ng Strap‐MO was injected unilaterally into two‐cell‐ stage embryos.
The embryos were examined at neurula stages 17‐18 by ISH for neural markers. E. 50 ng Strap‐MO was injected into
the animal regions of two‐cell‐stage embryos. Animal caps were dissected at blastula stage 9 and cultured until neu‐
rula stages 19‐20. RNA was then extracted for RT‐qPCR analysis of marker expression. Bars represent mean ±s.d., n=3.
* P<0.05, **P<0.01, when compared with the control. Image published in: Jin L et al. (2018) Copyright © 2018. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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