XB-IMG-172743
Xenbase Image ID: 172743
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Fig. 1. Comparative analysis of the temporal profile for pancreatic marker gene
expression in vivo and in programmed explants
(A) Ectodermal explants isolated from stage 8/9 embryos and injected as indicated were
treated with 5µM RA and cultivated until the equivalent of stage 30. Detection of marker gene
expression for the genes indicated was done by RT-PCR. (B) and (C) Transcript
quantification of whole embryos (5 embryos per condition) and explants (approximately 50
explants per condition) from two biological replicates with untreated and treated embryos
grown to the equivalent of the developmental stages indicated using Nanostring analysis (B)
and RT-PCR (C). Average values are given as mean and error bars as s.e.m.. E, embryo; C,
un-programmed explants; PE, pancreatic explants (VegT/Noggin/RA); øRA, programmed
explants with blocked endogenous RA-signalling (VegT/Noggin/Cyp26a1). Image published in: Gere-Becker MB et al. (2018) © 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |