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Fig. 2. Identification of Hnf1β and Fzd4 as direct endodermal RA-target genes (A) Experimental procedure for the identification of early direct RA-target genes in the context of pancreas development. (B) Venn diagram comparing genes differentially expressed within two hours after RA-addition in the absence or presence of CHX. 46 putative direct RA-target genes were induced under both conditions. (C) RNA-sequencing results for RA-mediated Hnf1β and Fzd4 induction. The number of mapped reads two hours after the addition of RA in the presence or absence of CHX is indicated. The data result from two biological replicates with approximately 50 explants per condition. Average values are given as mean and error bars as s.e.m.. (D) WMISH for Hnf1β and Fzd4 in gastrula stage embryos. Whole embryos are depicted on the left-hand side and bisected embryos on the right-hand side. dbl, dorsal blastopore lip; e, endoderm; m, mesoderm; ne, neuro-ectoderm Image published in: Gere-Becker MB et al. (2018) © 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue hnf1b.L hnf-1beta, hnf1-beta, hnf1beta, lfb3, tcf2, vhnf1 X. laevis Sometime during NF stage 10 to NF stage 12.5 endoderm upper blastopore lip fzd4.L frizzled 4, frizzled-4, frizzled4, Fz-4, Fz4, LOC108708760, Xfz4 X. laevis Sometime during NF stage 10 to NF stage 12.5 endoderm neuroectoderm upper blastopore lip Image source: Published Larger Image Printer Friendly View

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