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Fig. S6. Specificity of the Hnf1β morpholino antisense oligonucleotide (A) The Hnf1β morphilono antisense oligonucleotide (Hnf1b-mo) targets the intron1/exon2 boundary (E1/E2) of the Hnf1β pre-mRNA, resulting in the loss of exon 2, leading to a shortened open reading frame (ORF) lacking functional DNA-binding domains (POUs and POUh) and also lacking the transactivation domain. (B) The specificity of the Morpholino was tested in the explant system. RT-PCR with oligonucleotides bind to exon 1 and exon 3. A smaller Hnf1β amplicon is detected in the presence of the morpholino. (C) Sequence analysis of the shorter Hnf1β amplicon upon Hnf1b-mo application confirms the loss of exon 2. Image published in: Gere-Becker MB et al. (2018) © 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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