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Fig. S10. Mutation analysis for the genomic locus of Fzd4 exon1 and the putative offtarget Kremen2 in CRISPR/Cas-injected pancreatic explants DNA sequences of (A) Fzd4 and (B) Kremen2 amplicons from CRISPR/Cas injected pancreatic explants were aligned to genomic X.leavis Fzd4 and Kremen2 sequences. The Fzd4-gRNA target sequence is highlighted in yellow and the number of deleted nucleotides indicated.

Image published in: Gere-Becker MB et al. (2018)

© 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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