Xenbase Image ID: 172799

Fig. 3. Expression of X. laevis hnRNP E2 and abundance of the a and b transcripts during development. (A) 2 mg of poly(A) RNA from oocytes and embryos were analyzed on a Northern gel and hybridized with X. laevis hnRNP E2 and ribosomal protein L4 probes. (B) Southern blot analysis of RT-PCR products of total RNA from embryos of different stages obtained using the oligos indicated in Fig. 1 as described in Material and methods. PCR reactions were loaded on a 3.5% agarose gel, blotted and hybridized to a 50 labeled oligo which recognizes both a and b transcripts. The size of the two fragments on the gel matches the predicted sizes of the amplified regions, 248 nt for the a form and 146 for the b form. Molecular weight markers are indicated on the left. Image published in: Gravina P et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page