XB-IMG-172801
Xenbase Image ID: 172801
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Fig. 5. Nucleo-cytoplasm partition of hnRNP E2. (A) Extracts from manually
dissected oocyte nuclei and cytoplasms (the equivalent of four oocytes)
were analyzed by Western blot with an hnRNP E2 antiserum. Antibodies
were stripped from the filter which was then incubated with an antiserum
versus the X. laevis La antigen. (B) Cultured A6 cells were processed for
immunofluorescence as indicated in Material and methods, using X. laevis
hnRNP E2 affinity purified antibodies (top). Cells were transiently transfected
with the plasmid pCMV hnRNP E2 that encodes for myc-tagged
hnRNP E2 (bottom). Immunostaining was performed using the mAB 9E10,
that recognizes the myc region of the protein. The arrow points to a nontransfected
cell. Image published in: Gravina P et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |