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Supplementary Figure 3: Phenocopy of ruby mutant by pax8 MO injection. Pax8 morpholino was injected at one (a) or two cell stage (single cell injected) (b). Embryos were fixed at st 36-37 and pronephric development was assessed by pax2 expression. Abnormalities in pronephros development were classified: No phenotype; pronephros similar to uninjected controls (UIC); Type I: disrupted and abnormal pronephros similar to ruby mutants on both sides of the embryos. Type II: abnormal pronephros on one side and moderate abnormal pronephros on the other side; Type III: disrupted and abnormal pronephros on the injected side and moderate abnormal phenotype on the uninjected side. Type IV: disrupted and abnormal on the injected side and normal pronephros on the uninjected side. Images of Pax2 WMISH showing each type of phenotype are shown. For Type III and IV, embryos shown were injected on the right side. Arrowheads indicate disrupted and abnormal pronephros. Image published in: del Viso F et al. (2012) Copyright ©2012 del Viso et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pax2 LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. tropicalis Sometime during NF stage 35 and 36 to NF stage 37 and 38 pronephric kidney pronephric nephrostome pronephric duct pronephric tubule otic vesicle midbrain-hindbrain boundary hindbrain Image source: Published Larger Image Printer Friendly View

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