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Fig. S3. SMAD1 inhibition during cardiac chamber differentiation does not affect expression of the cardiac markers tropomyosin and Tbx5. (A,B) In situ hybridization for Tbx5 on stage 40 DMSO (A) and dorsomorphin-treated (B) anterior explants shows normal expression of Tbx5 in the heart and cardinal vein after SMAD1 inhibition. (C-F) Whole-mount antibody staining for tropomyosin of a stage 40 DMSO (C,E) or dorsomorphin-treated (D,F) anterior explants. Ventral view reveals no change in expression of tropomyosin in the heart after treatment with dorsomorphin. Dorso, dorsomorphin. Scale bars: 1 mm. Image published in: Mandel EM et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tbx5.L tbx-5, tbx5-a, tbx5-b, xtbx-5, xtbx5 X. laevis Throughout NF stage 40 heart cardinal vein tpm1.L alpha-tm, alpha-tropomyosin, CH1 X. laevis Throughout NF stage 40 heart Image source: Published Larger Image Printer Friendly View

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