Xenbase Image ID: 172913

Fig. 4. Spectrin βV associates with post-Golgi structures and binds to the Rab8 GTPase. (A) In mouse isolated vestibular hair cells (VHCs) on P10, the staining with TRITC-tagged wheat germ agglutinin (WGA) substantially overlaps with the spectrin βV immunostaining, both in the Golgi apparatus and in scattered post-Golgi secretory vesicles. (B) Focus on a spectrin βV-immunoreactive and TRITC-WGA-stained spot in the Golgi apparatus. (C) The TRITC-WGA–stained structures (red) are not colocated with spectrin αII- and spectrin βII-immunoreactive structures (green). (D) Unlike in VHCs, spectrin βV in mouse cochlear outer hair cells (OHCs) does not colocalize with TRITC-WGA–stained structures. (E) Spectrin βV binds to Rab8, a small GTPase involved in vesicular trafficking toward the plasma membrane. A GST-tagged R29-Cter fragment of spectrin βV (GST-βV R29-Cter), but not GST alone, binds to Rab8 present in protein extracts from the mouse inner ear or retina. Bars, 2 µm. Image published in: Cortese M et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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