Xenbase Image ID: 172914

Fig. 5. Spectrin family tree and signatures of positive selection in the amino acid sequences of mammalian spectrins βV. (A) Neighbor-joining phylogenetic tree of the complete spectrin gene family based on a multiple full-length sequence alignment from vertebrate and invertebrate species (Jones–Taylor–Thornton amino acid substitution model). Vertebrate spectrin branches were collapsed for the sake of clarity (see detailed tree in SI Appendix, Fig. S7). Predicted domains of the spectrins identified in the choanoflagellate Monosiga brevicollis are shown on the right side of the corresponding tree branches: MONBRDRAFT_14504, _31289, and _23028 refer to the putative α, β, and β-heavy (βH) spectrins, respectively. The nonconventional β spectrins of Drosophila (β-heavy) and mouse (βV) are shown for comparison. Bar, 0.5 substitution per site. (B) Diagram showing the positions of the amino acid residues submitted to positive selection in the mammalian lineage for spectrin βV (35 codon sites). Corresponding positions in the different vertebrate species are shown, with upper numbers indicating the positions in the human protein sequence. The protein domains are indicated below by horizontal bars. The phylogenetic tree of this spectrin is shown on the left side (the red and blue arrows indicate the mammalian and avian focal branches, respectively). Bar, 0.5 substitution per site. The detailed results of the phylogenic molecular analysis are presented in SI Appendix, Table S2. CH, calponin homology domain; Rn, spectrin repeat number n; PH, pleckstrin homology domain. Image published in: Cortese M et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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