Xenbase Image ID: 173140

Focus on selected structures. (A) Schematic of the Wnt reporter construct containing chicken β-globin insulators [20]. (B, C) Embryo transverse sections following whole-mount gfp in situ hybridization on Tg(pbin7Lef-dGFP) embryos, at stage 12.5 (B) and 22 (C). Right panels are enlargement of blue squares. Black dotted lines delineate the somites. (D) Whole-mount gfp in situ hybridization of Tg(pbin7Lef-dGFP) embryos at stages 29–30 and transversal sections at indicated level showing gfp staining in the dorsal and ventral fins. (E) Dorsal views of a stage 13 Tg(pbin7Lef-dGFP) embryo first hybridized with probe against gfp and secondarily with probe against sox2. Black dotted lines delineate the gfp-expressing domain. (F) Embryo transverse sections following whole-mount gfp in situ hybridization on Tg(pbin7Lef-dGFP) embryos, at stage 18 and 24–25. The squared regions delineated with the dotted line were enlarged. White dotted lines delineate the neural tube. (G) Dorsal view of a stage 12 Tg(pbin7Lef-dGFP) embryo hybridized with gfp and pax3 probes or gfp and otx2 probes (posterior side is up). Dotted lines delineate gfp staining. d-f: dorsal fins; ectod.: ectoderm; ins: fp: floorplate; insulator; mesod.: mesoderm; n: notochord; np: neural plate; NT: neural tube; v-f: ventral fins. Image published in: Borday C et al. (2018) © 2018 Borday et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 14 neural plate otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 12 anterior pax3.L cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 12 neuroectoderm Image source: Published Larger Image Printer Friendly View

Return to previous page