Xenbase Image ID: 173142

Fig. 1. Xenopus March2 interacts with Dsh. (A) Co-immunoprecipitation analysis with GFP-March2 and Myc-XDsh in HEK293FT cells. (B) Interaction of endogenous human Dvl1 with March2 in HEK293FT cells. Immunoprecipitation with anti-IgG antibody used as a negative control. (C) Xenopus animal caps injected with GFP-XDsh (500 pg) and HA-Mar2 (250 pg) mRNAs were immunostained and analyzed by confocal microscopy. (D) HeLa cells transfected with GFP-XDsh were stained with anti-GFP and anti-March2 antibody and analyzed by confocal microscopy. (E) Schematics of the various XDsh deletion mutants (top) used for co-immunoprecipitation analysis (bottom). Note that the PDZ and DEP domains of Dsh are required for the March2 interaction in HEK293FT cells. (F) Schematics of full-length March2, C-terminus-deleted and N-terminus-deleted mutant forms (top) and co-immunoprecipitation results (bottom) showing that the C-terminal region of March2 is important for the interaction with Dsh. Image published in: Lee H et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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