Xenbase Image ID: 173143

Fig. 2. Xenopus March2 induces the ubiquitylation-mediated degradation of Dsh. (A) March2 decreases Dsh protein in Xenopus. HA-Mar2 mRNA (250 pg, 500 pg, 1 ng) along with 200 pg of GFP-XDsh mRNA were injected and analyzed by western blotting at stage 10.5. (B) Mar2MO stabilizes Dsh protein. Mar2MO (40, 80 ng) was injected with 150 pg of GFP-XDsh mRNA. Co-injection of 250, 500 pg and 1 ng of March2 mRNA along with 80 ng of Mar2MO gradually reduced Dsh levels again. (C) DEP and PDZ domains of Dsh are required for March2 mediated Dsh degradation. All forms of GFPXDsh mRNA, 250 pg; HA-Mar2 mRNA, 1 ng. (D) March2CS cannot degrade Dsh. The amounts of mRNAs: Myc-XDsh, 250 pg; HAMar2 WT and CS, 500 pg and 1 ng, respectively. (E,F) In vivo ubiquitylation assays in Xenopus. Dsh protein was pulled down and poly-ubiquitylated Dsh protein was detected. The amounts of injected mRNAs: Flag-Ub, 2 ng; Myc-XDsh, 2 ng; GFP-Mar2 WT and CS, 1 ng; Mar2MO, 40 ng. Image published in: Lee H et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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