XB-IMG-173144
Xenbase Image ID: 173144
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Fig. 3. March2 mediates Dsh for the lysosomal degradation pathway. (A,B) In vivo ubiquitylation assays in Xenopus. XDsh was pulled down with anti-Myc antibody and its ubiquitylation was detected by anti-Flag antibody. Poly-ubiquitylated Dsh protein was increased by March2 with bafilomycin A1 (A) or leupeptin (B) treatment. (C) Confocal images of Xenopus tissues showing the subcellular localization of March2 protein. Animal caps injected with Myc-Mar2 mRNA (200 pg) were immunostained with anti-EEA1 or Rab7 antibodies and analyzed. White arrows indicate colocalized puncta of March2 with Rab7. (D) Confocal images of HeLa cells showing colocalization of March2 and Dpr1 in Lamp1-positive puncta (white arrows). Image published in: Lee H et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |