XB-IMG-173153
Xenbase Image ID: 173153
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Fig. 1. Interfering with G-protein binding of Pkd1 results in a PKD phenotype. (A-F) Xenopus embryos injected with Gnal-MO or Gnas-MO and uninjected
control embryos were analyzed morphologically and histologically at stage 42. (G) Schematic of the Pkd1 C-terminal tail (CTT) indicating the three
conserved domains: the G-protein binding (GBD), the PEST and the coiled-coil domain (CC). The percentage identity between the Xenopus and human protein
for each domain is indicated. (H-L) Uninjected controls and embryos injected with Pkd1-sMO alone or co-injected with Pkd1-CTT or Pkd1-CTTδGBD mRNA
were analyzed by Nbc1 whole-mount in situ hybridization at stage 39. The rescue of Nbc1 in the late distal tubule upon injection of the Pkd1-CTT mRNA is
indicated by red arrowheads. (L) Quantification of three independent experiments with white bars indicating normal expression, gray bars reduced expression and
black bars absent expression. The number of embryos examined is indicated above each bar. The data are presented as cumulative numbers of at
least three biological replicas. Image published in: Zhang B et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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