XB-IMG-173155
Xenbase Image ID: 173155
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Fig. 3. Surface plasmon resonance
analysis of the interaction between
G-protein α subunits with the
polycystin 1 C-terminal tail. (A,A′) SPR
analysis between the C-terminal tail of
Pkd1 (Pkd1-CTT) and the different
G-protein α subunits expressed in the
pronephros of Xenopus embryos. (A) A
representative sensorgram and (A′) a
table of the equilibrium dissociation
constants [KD (M)] for each interaction.
(B,B′) SPR analysis between G-protein α
subunits and Pkd1-CTT or Pkd1-
CTTΔGBD proteins. (B) A representative
sensorgram and (B′) a table of the
equilibrium dissociation constants [KD (M)]
for each interaction. This analysis not only
includes the Xenopus G-protein α
subunits with high binding affinity, but also
mouse Gna12, which has been shown to
interact with Pkd1 (Parnell et al., 1998;
Yuasa et al., 2004). Image published in: Zhang B et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |