XB-IMG-173158
Xenbase Image ID: 173158
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Fig. 6. The Pkd1 mutant mouse proximal tubular cells
upregulation GPCR signaling. (A-J) 3D-Matrigel assay
(A-H) and growth curves (I,J) of the isogenic Pkd1Fl/− and
Pkd1−/− cells in the presence or absence of 5 µM H89, 5 µM
gallein or a combination of both. (K,L) Dose-response curves
for the inhibition of cAMP production by somatostatin (K) or the
activation of it by parathyroid hormone (PTH) (L) comparing
Pkd1Fl/− and Pkd1−/− cells. (M) Western blot analysis of
immunoprecipitated Gnas using both a total and an activated
Gnas antibody demonstrates increased levels of GTP-bound
Gnas in response to 10−6 M PTH. (N) Western blot analysis
examining total Gnas levels between the two cell lines. Image published in: Zhang B et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |