XB-IMG-173160
Xenbase Image ID: 173160
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Figure S2: Conservation of the C-terminal Tail of Pkd1. (A) ClustalW alignment of the
intracellular domain of Polycystin-1 protein from human (GenBank Accession #NP_000287.3)
and Xenopus tropicalis (GenBank Accession #XP_002932517.1). Four previously described
domains were analyzed. The G-protein binding signature (GBD) is based on the analysis by
Parnell et al. (1998). It is indicated in yellow and the domain-identifying amino acids
(BB….BBxxB) are bolded. This sequence overlaps with the Nuclear Localization Sequence
(NLS) described in Chauvet et al. (2004) and is indicated by a black line above the human
sequence. The PEST sequences were identified using epestfind. The optimal and predicted
domains are indicated in green and turquoise, respectively; the key amino acids identified in
Low et al. (2006) are indicated in bold. The coiled-coil domain were identified using Paircoil2
and are labeled in red. (B,C) Immunofluorescence analysis of HEK293T cells transiently
transfected with Pkd1-CTT-Myc (B) or Pkd1-CTTΔGBD-Myc (C). Nuclei were counterstained
with DAPI (blue). Insets show close-up of individual cells. Image published in: Zhang B et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |