Xenbase Image ID: 173168

Fig. 4 Effects of Six1 knockdown on early ectodermal TFs. a–j Expression of PPE (Eya1, Sox3), NC (FoxD3), neural plate (Sox3) markers and early ectodermal TFs in dorsal views of neural plate stage Xenopus embryos after injection of Six1 MO1 + MO2. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by blue LacZ staining). Reductions (arrows) and increased or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Green lines highlight broadening of the neural plate and lateral displacement of NPB markers on the injected side (bright green) versus control side (dark green). Insets show alternative phenotypes. k Summary of regulatory interactions. Arrows indicate requirement of Six1 for expression of TFs (reduction after Six1 knockdown). Bars indicate requirement of Six1 for restriction of expression of TFs (increase after Six1 knockdown). Faint colors indicate less frequent phenotypes. See Additional file 1: Table S8 for numbers. Image published in: Maharana SK and Schlosser G (2018) © Schlosser et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue eya1.L eyes absent-1, XEya1 X. laevis Sometime during NF stage 13 to NF stage 21 preplacodal ectoderm sox3.L Sry, Xlsox3, xSox3, Xtsox3 X. laevis Sometime during NF stage 13 to NF stage 21 preplacodal ectoderm neural plate foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Sometime during NF stage 13 to NF stage 21 neural crest zic1.L odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Sometime during NF stage 13 to NF stage 21 neuroectoderm neural plate neural crest pax3.L cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Sometime during NF stage 13 to NF stage 21 neural plate border neuroectoderm tfap2a.L ap2, AP2a, AP2alpha, tfap2a-a, tfap2a-b, tfap2alpha, XAP-2, xap2 X. laevis Sometime during NF stage 13 to NF stage 21 neuroectoderm neural plate border neural crest non-neural ectoderm msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Sometime during NF stage 13 to NF stage 21 neural plate border anterior placodal area foxi4.2.L foxi1a, foxi4.2-a, foxi4.2-b, foxia, XFD2, Xfoxi1, Xfoxi1a, xlFox1a X. laevis Sometime during NF stage 13 to NF stage 21 preplacodal ectoderm dlx3.L ai4, dlx3-a, dlx3-b, tdo, Xdll-2, xdlx3 X. laevis Sometime during NF stage 13 to NF stage 21 non-neural ectoderm gata2.L GATA-2, xGATA-2, xgata2 X. laevis Sometime during NF stage 13 to NF stage 21 non-neural ectoderm Image source: Published Larger Image Printer Friendly View

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